Beacon Cholesterol

CHOLESTEROL
INTENDED USE: The reagent kit is intended for the “in vitro” quantitative
determination of Cholesterol in serum/plasma.

CLINICAL SIGNIFICANCE:
Cholesterol is the main lipid found in blood, bile and brain tissue. 
Itis the main lipid associated with arteriosclerotic vascular
diseases.

It is required for the formation of steroids and cellular
membranes. The liver metabolizes the cholesterol and it is
transported in the blood stream by lipoproteins. 
Increased levels are found in hypercholesterolaemia , hyperlipidaemia
hypothyroidism, uncontrolled diabetes, nephrotic syndrome and
cirrhosis. Decreased levels are found in malabsorption,
malnutrition, hyperthyroidism anemias and liver diseases.

PRINCIPLE:
Cholesterol esterase hydrolyses esterified cholesterols to free
cholesterol. The free cholesterol is oxidized to form hydrogen
peroxide which further reacts with phenol and 4-aminoantipyrine
by the catalytic action of peroxidase to form a red coloured
quinoneimine dye complex. 
Intensity of the colour formed is directly proportional to the 
amount of cholesterol present in the sample.

Cholesterol Esterase
Cholesterol esters + H O Cholesterol + Fatty 2
Acids
Cholesterol Oxidase
Cholesterol + O2
Cholestenone + H2O2
H O + Phenol + 4 amino antipyrine Red quinonimine 2 2
dye + H2O </sp

CONTENTS: 
Reagent 1 : Cholesterol Enzyme Reagent
Reagent 2 : Cholesterol Standard 200 mg/dl. 
Reagent 3 : Cholesterol Precipitating Reagent (Free)